Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
Isis Innovation Limited
This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.
1ClaimsFirst of 8
- 1
A method for generating an array of oligonucleotides of chosen lengths within discrete cells of a support material comprising the steps of a) segregating a support material into discrete cell locations; b) coupling a nucleotide precursor to a first set of cell locations; c) coupling a nucleotide precursor to a second set of cell locations; d) coupling a nucleotide precursor to a third set of cell locations; e) and continuing the sequence of coupling steps until the desired array has been…
2Description
This application is a continuation of now abandoned Ser. No. 07/695,682, filed May 3, 1991, abd, which is a continuation-in-part of abandoned Ser. No. 07/573,317, filed Sep. 28, 1990, abd, which is the U.S. National Stage of PCT/GB89/00460, filed May 2, 1989.