Patent
Cryopreservation of swine embryos (US Patent 6503698)
The United States of America as represented by the Secretary of Agriculture · Granted Jan 7, 2003 · Filed Jun 16, 2000
Patent number: 6503698 Kind code: B1 Granted: 2003-01-07 Filed: 2000-06-16 Inventors: John R. Dobrinsky (Beltsville, MD); Hiroshi Nagashima (Kawasaki, JP) Assignees: The United States of America as represented by the Secretary of Agriculture (Washington, DC)
Abstract
A method for the cryopreservation of zona pellucida-intact, early-stage swine embryos, morulae and blastocysts includes centrifuging the embryos prior to cryopreservation in order to remove intracytoplasmic lipids. The lipids are localized external to the embryo proper
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