Preparation of 13C/15N-labeled oligomers using the polymerase chain reaction (US Patent 6258567)

Patent number: 6258567 Granted: 2001-07-10 Filed: 1999-05-29 Inventors: Xian Chen (Los Alamos, NM, US); Goutam Gupta (Santa Fe, NM, US); E. Morton Bradbury (Santa Fe, NM, US) Assignees: The Regents of the University of California (Los Alamos, NM, US)

Abstract

Preparation of .sup.13 C/.sup.15 N-labeled DNA oligomers using the polymerase chain reaction (PCR). A PCR based method for uniform (.sup.13 C/.sup.15 N)-labeling of DNA duplexes is described. Multiple copies of a blunt-ended duplex