Preparation of Forssman antibody neutralizing antigen and product thereof
Joseph Zichis, deceased and Lillian K. Zichis, executrix
Three antigens, termed HA, MN and FN, are prepared for the serological diagnosis of infectious mononucleosis; the HA antigen being used to identify the heterophil antibodies and the FN and MN antigens are used to differentiate them.
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The method of preparing a Forssman antibody neutralizing antigen, FN, composition comprising storing comminuted guinea pig or horse kidney tissue in saline solution containing NaN.sub.3 at from about 2.degree. to 4.degree. C. for about 24 hours, heating the resulting suspension to about 95.degree. C. and maintaining it at that temperature for about one-half hour, separating the solids from the supernatant liquid and cooling the liquid to about 25.degree. C., adjusting the pH of the cooled…
2Description
This is a division of application Ser. No. 689,308, filed May 24, 1976, now U.S. Pat. No. 4,139,606.