Purified thermostable pyrococcus furiosus DNA polymerase I (US Patent 5948663)

Patent number: 5948663 Granted: 1999-09-07 Filed: 1991-12-02 Inventors: Eric J. Mathur (Solana Beach, CA, US) Assignees: Stratagene (San Diego, CA, US)

Abstract

Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000-93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000