Extraction and purification of an anticoagulant principle from the south american leech, haementeria ghilianii (US Patent 4832849)

Patent number: 4832849 Granted: 1989-05-23 Filed: 1988-06-16 Inventors: Alan D. Cardin (Cincinnati, OH, US) Assignees: Merrell Dow Pharmaceuticals Inc. (Cincinnati, OH, US)

Abstract

Applicant has isolated a Factor X.sub.a inhibiting substance from the salivary gland extract of the leech H.ghilianii. The extract is subjected to separation using both DEAE-cellulose and heparin-agarose chromatography resins eluting with an increasing salt gradient. The