Application of Floyd Stephen Markham and Herald Rea Cox

WORLEY, Chief Judge.

Markham and Cox appeal from the decision of the Board of Appeals affirming the examiner’s rejection of claims 8, 14, 15 and 16 of their patent application1 which relates to a process of preparing a *359live virus vaccine for various fowl diseases, particularly Newcastle disease, a virus disease of chickens.

Claim 14 reads:

“14. A method of preparing a live-virus fowl vaccine, said vaccine being free of the microorganism causing chronic respiratory disease of fowl, which comprises the step of preparing an aqueous suspension of chick embryo tissue in which live virus is present,…